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神经递质检测系统

  • 更新时间:2023-08-22
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简要描述:神经递质实时检测系统应用快速扫描循环伏安法(FSCV)技术,快速实时监测动物体内的儿茶酚胺类神经化学物质的含量(如多巴胺,,血清素等)

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神经递质实时检测系统应用快速扫描循环伏安法(FSCV)技术,快速实时监测动物体内的儿茶酚胺类神经化学物质的含量(如多巴胺,,血清素等)。系统使用了碳纤维生物传感器,可在大小鼠身体上实现短期的快速测量。


系统有两种款式:一种为用于大鼠的无线遥测系统,一种为应用到小鼠的有线遥测系统。


技术优势:
√ 良好的空间分辨率-直径34um 碳纤维
√ 良好的时间分辨率- 5-10 sweeps/s
 准确度-使用现有的可定义的明确儿茶酚胺类标记物,排除了其他种类物质的干扰。
√ 采样速率: 5k/s (大鼠系统),或者10K/s(小鼠系统)

工作原理:
生物胺类物质的含量通过快速循环性的施加电压到一根植入式碳纤维传感器,然后检测相应的产生的电流变化进行测定。系统使得在进行详细的行为学研究的同时,可以测量自发产生的亚秒级的神经递质释放事件。无论是有线还是无线系统,都可以在可选的量程(-0.6-+1.5V)范围内,进行250-400V/s的扫描检测。所有系统同时还内置支持外部刺激的控制。


软件:

系统配套软件不仅支持传统的短期测量模式(记录2分钟以内的数据),同时还支持扩展的连续长期记录模式。除此之外,本软件的特点还包括背景噪音消除,热点图,3D可视化视图,可选的滤波以及动态的伏案图。数据可以导出为通用的EXCEL格式文件。另外,软件还支持整合同步视频捕捉,以便适合于动物行为与生物物质释放关系的同步研究。


FAST SCAN CYCLIC VOLTAMMETRY SYSTEM

Pinnacle’s FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is specifically designed to simplify the measurement of catecholamines (i.e., dopamine, norepinephrine, and serotonin). Pinnacle offers turn-key systems for both mice and rats. Both the wireless rat system and the tethered mouse system use carbon fiber sensors to enable short-term measurement in the brains of freely moving animals.





HOW IT WORKS

Biogenic amine levels are detected by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resultant current. Our systems can measure spontaneous sub-second neurotransmitter release events while conducting detailed behavioral studies. Both the wireless and tethered systems sweep from 250 to 400 V/s in a user-selectable range spanning -1.1 to +1.3 V. All systems have built-in support for controlling an external stimulus.

l  Voltage Span: -1.1 V to +1.3 V

l  Range: 250 – 400 V/s 

l Sweeps/second: 5 - 10 

l Points/sweep: 1000 



Analysis

Dopamine (blue), serotonin (green), and norepinephrine (pink) have specific voltammogram profiles when detected by FSCV.


The TETHERED FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM allows researchers to harness the powerful genetics of the mouse model to address underlying mechanisms of biogenic neurotransmitter release and function. A headmounted FSCV board sends signals through a low-torque commutator to an interface box that streams data to the host PC. The system comes with Pinnacle’s FREE 8500 software.



1. The FSCV interface box provides access to stimulus lines and transmits data to PAL-8500 software.

2. A low-torque commutator, which is mounted above the cage, allows for unencumbered freedom of movement.

3. Signals are amplified and filtered at the head of the animal using our headstage, which ensures the delivery of clean, artifact-free data.

4. Stereotaxically placed guide cannulas allow easy insertion of carbon fiber sensors. Headmounts are affixed to the skull with dental acrylic and act as a connection port for the headstage.

 




CARBON FIBER


Pinnacle’s FSCV system uses CARBON FIBER SENSORS (CFSs) to measure the presence of dopamine and other catecholamines in the brains of freely moving animals. Our sensor is a 34 µm diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica. All Pinnacle CFSs require an Ag/AgCl reference electrode.


Our sensor is a 34 um diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica.

 




CARBON FIBER SENSORS

Carbon fiber sensors are ordered by cannula type and whether the researcher needs to remove them from the cannula for post-calibration. CFS-F sensors are fixed in the cannula and cannot be removed for post-calibration。



FSCV PAL-8500 SOFTWARE PACKAGE

The FSCV system includes Pinnacle’s FREE PAL-8500 software, which supports traditional, short recording paradigms (recordings of two minutes or less) as well as longer-term recordings that use an extended continuous mode. Furthermore, the suite supports integrated, synchronized video recording, which allows monitoring of animal behavior simultaneously with biogenic amine release.

 


Additional features of this software include:
• Background Subtraction
• 3D Visualization 
• User-Selectable Filters 
• Heat Maps 
• Animated Voltammograms
• Export to Third-Party Packages 



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